Generator

Part:BBa_K1403003

Designed by: Sylvia   Group: iGEM14_Paris_Bettencourt   (2014-10-06)


Paris Bettencourt 2014 Odor Palette

ODOR WHEEL.png

Our odor palette is composed of BioBricks containing codon-optimized sequences of enzymes known to catalyze reactions that yield volatile compounds with distinctive smells.

We included odors with different tonalities in order to explore the aromas resulting from combinations of smelly units in E. coli. The tonality of each smell is categorized as butter, balminess, citrus, non-citrus fruit and herbal. These cover half of the main odor categories perceivable to human beings.

Each part has a standardized architecture consisting on a constitutive promoter and a functional RBS. The palette could be used as an educational tool to introduce synthetic biology concepts to the general public.

SystemEnzymeSubstrateConcentrationProduct
Jasmine
BBa_K1403006
JMTJasmonic acid1 mMMethyl jasmonate
Mint
BBa_K1403009
BMST1Salicylic acid2 mMMethyl salicylate
Banana
BBa_K1403012
ATF1Isoamyl alcohol5 mMIsoamyl acetate
Limonene
BBa_K1403003
LIMS1Farnesyl diphosphateNatural(+)-Limonene
Rain
BBa_K1403013
GDSFarnesyl diphosphateNaturalGeosmin
Flowers
BBa_K1403009
BMST1Benzoic acid5 mMMethyl benzoate
Butter
BBa_K1403017 & BBa_K1403019
AldB
ilvB/N
Acetolactic acid
Pyruvate
NaturalAcetoin
Sweat
BBa_K1403002
agaA3-M-2-hexenoic acid-3-HO-3-Methylhexanoic acid

Limonene synthase (LIMS1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: LIMS1 from Citrus limon

Transform E. coli for (+)-Limonene production.
Click on Design tab for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Verification

Limonene.png

Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator

[edit]
Categories
Parameters
None