Difference between revisions of "Template:TypeIIS/Plasmids/Level1"
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| − | When four Level 0 parts are assembled into the | + | When four Level 0 parts are assembled into the plasmid backbone (with <partinfo>BBa_J04454</partinfo>), the resulting transcriptional unit (TU) will be flanked by SapI restriction sites and the specified 3 bp Fusion Sites (NNN), which can be used for subsequent (Level 2) assemblies. |
<center>[[File:TypeIIS-Level1-L0-Part-Assembly-Simple.png|900px]]</center> | <center>[[File:TypeIIS-Level1-L0-Part-Assembly-Simple.png|900px]]</center> | ||
Revision as of 20:20, 17 June 2020
Simplified diagram of BBa_J04454 and pSB1K0# with BsaI cut site.
When four Level 0 parts are assembled into the plasmid backbone (with BBa_J04454), the resulting transcriptional unit (TU) will be flanked by SapI restriction sites and the specified 3 bp Fusion Sites (NNN), which can be used for subsequent (Level 2) assemblies.
Level 1 Plasmid Set
Level 1 transcriptional units in this set of plasmid backbones will be flanked by SapI recognition sites and fusion sites, enabling further assembly of up to 4 transcriptional units as a Level 2 Assembly.
| Registry Name | Loop Name | Fusion Site 5' | TU | Fusion Site 3' |
|---|---|---|---|---|
| pSB1K01 | pOdd1 | ATG | TU 1 | GCA |
| pSB1K02 | pOdd2 | GCA | TU 2 | TAC |
| pSB1K03 | pOdd3 | TAC | TU 3 | CAG |
| pSB1K04 | pOdd4 | CAG | TU 4 | GGT |
Note:
- Since pSB1K3 is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies.
- Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04454 have been verified.