Difference between revisions of "Template:TypeIIS/Plasmids/Level1"

Revision as of 20:52, 16 June 2020

When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with BBa_J04454), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.

Since pSB1K3 is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies.

Registry Name	Loop Name	Fusion Site 5'	TU	Fusion Site 3'
pSB1K01	pOdd1	ATG	TU 1	GCA
pSB1K02	pOdd2	GCA	TU 2	TAC
pSB1K03	pOdd3	TAC	TU 3	CAG
pSB1K04	pOdd4	CAG	TU 4	GGT

Note: Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04454 have been verified.

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	When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with <partinfo>BBa_J04454</partinfo>), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.		When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with <partinfo>BBa_J04454</partinfo>), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.