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Date Name Thumbnail Size Description Versions
11:56, 21 October 2019 Aptazyme Gua 1.jpg (file) 55 KB   2
11:54, 21 October 2019 Aptazyme Gua 0.jpg (file) 41 KB   2
11:52, 21 October 2019 Aptazyme Theo 5.jpg (file) 59 KB   2
11:52, 21 October 2019 Aptazyme Theo 2,5.jpg (file) 59 KB   2
11:50, 21 October 2019 Aptazyme Theo 0.jpg (file) 42 KB   2
18:34, 16 October 2019 Theo gel-2.jpg (file) 174 KB   3
18:31, 16 October 2019 Gua gel-2.jpg (file) 195 KB   1
18:15, 16 October 2019 Theo gel-1.jpg (file) 149 KB   4
18:23, 15 October 2019 Aptazyme off process.jpg (file) 415 KB   3
07:28, 15 October 2019 BasicPartsimg1.jpg (file) 309 KB Reverted to version as of 07:47, 14 October 2019 5
15:29, 14 October 2019 CharacK7315202.jpg (file) 203 KB Figure 2. GFP expression levels depending to IPTG concentration. The basic levels of GFP were calculated from BBa_K731520 characterization, n = 3. Equation applicated for establish this relation is y = 0,7206x + 7410,6. 1
15:28, 14 October 2019 CharacK731520.jpg (file) 93 KB Figure 1. iGEM Strasbourg team establish the kinetics of LacI-LacIQ promoter (BBa_K731300) induction by measuring GFP expression (BBa_E0040). Three different clones of XL1 Blue cells containing BBa_K731520 part were incubated overnight under rotation a... 1
11:10, 14 October 2019 Gua gel-1.jpg (file) 115 KB   1
10:17, 14 October 2019 Aptazyme process.jpg (file) 63 KB This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand. 1
09:25, 14 October 2019 Aptazyme switch-on process.jpg (file) 142 KB Figure 2: Schematic graphic of Guanine aptazyme switch-on based bacterial three-hybrid system 1
09:23, 14 October 2019 Aptazyme inactive process.jpg (file) 61 KB Schematic graphic of inactive aptazyme. Inactive aptazymes (lost self-cleavage capability) active BH3 system independently of ligand presence 1
09:16, 14 October 2019 Aptazyme switch-off process.jpg (file) 139 KB Schematic graphic of Theophylline aptazyme switch-off based bacterial three-hybrid system 1
09:13, 14 October 2019 Tout Ms2-MS2-Aptazyme-PP7-PP7.jpg (file) 52 KB   2
09:00, 14 October 2019 MS2-LexA et PP7-LexA.jpg (file) 159 KB   2
08:33, 14 October 2019 Lex A normal et LexA mut.jpg (file) 55 KB Overview of LexA heterodimer repress system. The figure is reproduced from [Daines, D. A., Granger-Schnarr, M., Dimitrova, M., & Silver, R. P. (2002). Use of LexA-based system to identify protein-protein interactions in vivo. Methods in Enzymology, 153... 2
08:17, 14 October 2019 Gua active et inactive.jpg (file) 247 KB A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). Figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S.... 3