Difference between revisions of "Part:pSB1C3"
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The distribution kits contain many samples of pSB1C3 as it is the Registry's main backbone for shipping and maintaining samples. Our recommendation when cloning a part into pSB1C3 is that you can use either linearized plasmid backbone provided in the kits, or pSB1C3 with a reporter (BBa_J04450). | The distribution kits contain many samples of pSB1C3 as it is the Registry's main backbone for shipping and maintaining samples. Our recommendation when cloning a part into pSB1C3 is that you can use either linearized plasmid backbone provided in the kits, or pSB1C3 with a reporter (BBa_J04450). | ||
| − | '''2017 Kit Plate 4 - Well | + | '''2017 Kit Plate 4 - Well 4B''' has a sample of BBa_J04450 in pSB1C3. |
Revision as of 20:40, 28 September 2017
High copy BioBrick assembly plasmid
pSB1C3 is a high copy number plasmid (RFC [10]) carrying chloramphenicol resistance.
The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
pSB1C3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
pSB1C3 is the designated Registry shipping plasmid backbone. All submissions for iGEM competitions must be submitted using pSB1C3.
Go to this page for more information on how to ship submissions to iGEM HQ.
Sample Location
The distribution kits contain many samples of pSB1C3 as it is the Registry's main backbone for shipping and maintaining samples. Our recommendation when cloning a part into pSB1C3 is that you can use either linearized plasmid backbone provided in the kits, or pSB1C3 with a reporter (BBa_J04450).
2017 Kit Plate 4 - Well 4B has a sample of BBa_J04450 in pSB1C3.
Usage and Biology
In Spring 2011, pSB1C3 was sequenced using several primers: Pre-R, Suff-F, VF2, VR, and internal primers for the origin and resistance. The reference sequence below matches the most recent sequencing verification of pSB1C3 (w/ BBa_J04450, located in SP 4000 Well 2A).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2049
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2055 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2049
Illegal XhoI site found at 1033
Illegal XhoI site found at 1925 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2049
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2049
Plasmid lacks a suffix.
Illegal XbaI site found at 2064
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.