Difference between revisions of "Part:pSB1AT3"

Revision as of 03:43, 9 June 2007

High copy BioBrick assembly plasmid

pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AT3

Plasmid map for pSB1AT3

Usage and Biology

Tetracycline resistant up to 150 ug/ml.

Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal NheI site found at 1270
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3431
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3425
Illegal BamHI site found at 1416
Illegal XhoI site found at 1035
Illegal XhoI site found at 2318
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3425
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3425
Plasmid lacks a suffix.
Illegal XbaI site found at 3440
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1442
Illegal NgoMIV site found at 1810
Illegal NgoMIV site found at 1970
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2464

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>pSB1AT3 short</partinfo>
 pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
+Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for [https://static.igem.org/mediawiki/parts/b/b0/PsB1AT3.ogg pSB1AT3] [[Image:PsB1AT3 map.jpg|thumb|center|Plasmid map for pSB1AT3]]
 ===Usage and Biology===