Difference between revisions of "Part:pSB1AC3"

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pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
 
pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
Annotated APE file for [https://static.igem.org/mediawiki/parts/9/98/PsB1AC3.ogg pSB1AC3] [[Image:PsB1AC3 map.jpg|thumb|center|pSB1AC3 Plasmid map]]
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Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for [https://static.igem.org/mediawiki/parts/9/98/PsB1AC3.ogg pSB1AC3] [[Image:PsB1AC3 map.jpg|thumb|center|pSB1AC3 Plasmid map]]
  
  

Revision as of 03:28, 9 June 2007

High copy BioBrick assembly plasmid

pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AC3
pSB1AC3 Plasmid map


Usage and Biology

Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3034
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3040
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3034
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3034
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3034
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2073