Difference between revisions of "Part:BBa K950011"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K950011 short</partinfo>
 
<partinfo>BBa_K950011 short</partinfo>
  
 
pRS316 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the URA3 gene for selection.
 
pRS316 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the URA3 gene for selection.
 +
 +
Team Tuebingen 2015 decided to make this vector backbone Biobrick compatible (RFC10). You can find that part here: [[Part:BBa_K1680016]].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:37, 26 September 2015

pRS316 shuttle vector

pRS316 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the URA3 gene for selection.

Team Tuebingen 2015 decided to make this vector backbone Biobrick compatible (RFC10). You can find that part here: Part:BBa_K1680016.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2047
    Illegal XbaI site found at 2017
    Illegal SpeI site found at 2023
    Illegal PstI site found at 389
    Illegal PstI site found at 2041
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2047
    Illegal SpeI site found at 2023
    Illegal PstI site found at 389
    Illegal PstI site found at 2041
    Illegal NotI site found at 2009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2047
    Illegal BamHI site found at 2029
    Illegal XhoI site found at 2080
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2047
    Illegal XbaI site found at 2017
    Illegal SpeI site found at 2023
    Illegal PstI site found at 389
    Illegal PstI site found at 2041
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2047
    Illegal XbaI site found at 2017
    Illegal SpeI site found at 2023
    Illegal PstI site found at 389
    Illegal PstI site found at 2041
    Illegal NgoMIV site found at 1668
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1079
    Illegal BsaI.rc site found at 3453
    Illegal SapI site found at 2370
    Illegal SapI site found at 4474
    Illegal SapI.rc site found at 926