Difference between revisions of "Part:BBa K950008"
 
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pRS313 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the HIS3 gene for selection.
 
pRS313 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the HIS3 gene for selection.
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Team Tuebingen 2015 decided to make this vector backbone Biobrick compatible (RFC10). You can find that part here: [[Part:BBa_K1680014]].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:32, 26 September 2015

pRS313 shuttle vector

pRS313 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the HIS3 gene for selection.

Team Tuebingen 2015 decided to make this vector backbone Biobrick compatible (RFC10). You can find that part here: Part:BBa_K1680014.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2127
    Illegal XbaI site found at 2097
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2127
    Illegal NheI site found at 1007
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
    Illegal NotI site found at 2089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2127
    Illegal BglII site found at 898
    Illegal BglII site found at 958
    Illegal BamHI site found at 2109
    Illegal XhoI site found at 2160
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2127
    Illegal XbaI site found at 2097
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2127
    Illegal XbaI site found at 2097
    Illegal SpeI site found at 2103
    Illegal PstI site found at 1182
    Illegal PstI site found at 2121
    Illegal NgoMIV site found at 1748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3533
    Illegal SapI site found at 2450
    Illegal SapI site found at 4554