Difference between revisions of "Part:BBa K935005:Experience"

 
(Applications of BBa_K935005)
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
===Applications of BBa_K935005===
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We transformed E.coli with a series of our arsenic responsive constructs.  BBa_K935005 contains three ArsR genes under the control of the pArs promoter.  We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs and essentially the same as that of BBa_K935004, which contains two ArsR genes.  In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.
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[[Image:BBa_K935004_and_BBa_K935005__Response_to_Arsenate.jpg]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 15:39, 6 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

We transformed E.coli with a series of our arsenic responsive constructs. BBa_K935005 contains three ArsR genes under the control of the pArs promoter. We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs and essentially the same as that of BBa_K935004, which contains two ArsR genes. In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.

BBa K935004 and BBa K935005 Response to Arsenate.jpg

User Reviews

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