Difference between revisions of "Part:BBa K935004:Experience"

(Applications of BBa_K935004)
 
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We transformed E.coli with a series of our arsenic responsive constructs.  BBa_K935004 contains two ArsR genes under the control of the pArs promoter.  We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs.  In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.
 
We transformed E.coli with a series of our arsenic responsive constructs.  BBa_K935004 contains two ArsR genes under the control of the pArs promoter.  We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs.  In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.
  
[[File:BBa_K935004_and_BBa_K935005__Response_to_Arsenate.jpg]]
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[[Image:BBa_K935004_and_BBa_K935005__Response_to_Arsenate.jpg]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 15:38, 6 October 2012

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We transformed E.coli with a series of our arsenic responsive constructs. BBa_K935004 contains two ArsR genes under the control of the pArs promoter. We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs. In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.

BBa K935004 and BBa K935005 Response to Arsenate.jpg

User Reviews

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