Difference between revisions of "Part:BBa K935002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Linked generators, one of ArsR under pArs and a separate RFP under pArs. The basal transcription rate of ArsR was found to raise the threshold of the production of RFP, essentially setting or "tuning" the latter generator. See related parts BBa_K935004 and BBa_K935005. | + | Linked generators, one of ArsR under pArs and a separate RFP under pArs. The basal transcription rate of ArsR was found to raise the threshold of the production of RFP, essentially setting or "tuning" the latter generator. See related parts BBa_K935004 and BBa_K935005. This device was built as a control for the function of BBa-J33201 in BBa_J61002. |
===Source=== | ===Source=== | ||
Latest revision as of 15:12, 3 October 2012
E. coli ArsR under the control of pArs coupled with pArs + RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1316
Illegal AgeI site found at 1428 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Linked generators, one of ArsR under pArs and a separate RFP under pArs. The basal transcription rate of ArsR was found to raise the threshold of the production of RFP, essentially setting or "tuning" the latter generator. See related parts BBa_K935004 and BBa_K935005. This device was built as a control for the function of BBa-J33201 in BBa_J61002.
Source
BBa_J33201 and BBa_K190015 with some modifications