Difference between revisions of "Part:BBa K935002"

 
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<partinfo>BBa_K935002 short</partinfo>
 
<partinfo>BBa_K935002 short</partinfo>
  
This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter.  This composite part was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction of RFP Expression. pArs-RFP was acquired from BBaK190015 and therefor there is no scar between the promoter and reporter allowing for an reporter substitution.
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This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter.  This device was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction of RFP expression. pArs-RFP was acquired from BBaK190015 and therefor there is no scar between the promoter and reporter allowing for a reporter substitution.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:14, 3 October 2012

E. coli ArsR under the control of pArs coupled with pArs + RFP

This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter. This device was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction of RFP expression. pArs-RFP was acquired from BBaK190015 and therefor there is no scar between the promoter and reporter allowing for a reporter substitution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1316
    Illegal AgeI site found at 1428
  • 1000
    COMPATIBLE WITH RFC[1000]