Difference between revisions of "Part:BBa K900001"
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PAGFP is a photoactivatable green fluorescent protein. PAGFP remains unactivated when exposed to 450-550nm light but fluoresces when exposed to a wavelength of 400 nm. It is used a reporter tag; by expressing the protein in cells or localizing to sub-cellular locations, one can gain temporal and spatial information within the cell by tracking organelles, proteins, and the whole cell itself. One can visualize the PAGFP with the same filter sets used for other green fluorescent proteins. | PAGFP is a photoactivatable green fluorescent protein. PAGFP remains unactivated when exposed to 450-550nm light but fluoresces when exposed to a wavelength of 400 nm. It is used a reporter tag; by expressing the protein in cells or localizing to sub-cellular locations, one can gain temporal and spatial information within the cell by tracking organelles, proteins, and the whole cell itself. One can visualize the PAGFP with the same filter sets used for other green fluorescent proteins. | ||
| − | [[Image: | + | [[Image:yVY027pre2.jpg|400px]] |
| − | [[Image: | + | [[Image:yVY027post2.jpg|400px]] <br> |
In these images, PAGFP is targeted to the peroxisome. The left image is prior to photoactivation and the right image is post activation. | In these images, PAGFP is targeted to the peroxisome. The left image is prior to photoactivation and the right image is post activation. | ||
Latest revision as of 03:17, 4 October 2012
PAGFP
PAGFP is a photoactivatable green fluorescent protein. PAGFP remains unactivated when exposed to 450-550nm light but fluoresces when exposed to a wavelength of 400 nm. It is used a reporter tag; by expressing the protein in cells or localizing to sub-cellular locations, one can gain temporal and spatial information within the cell by tracking organelles, proteins, and the whole cell itself. One can visualize the PAGFP with the same filter sets used for other green fluorescent proteins.
In these images, PAGFP is targeted to the peroxisome. The left image is prior to photoactivation and the right image is post activation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]