Difference between revisions of "Part:BBa K900000"
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PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350–400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag; by expressing in cells and localizing it to sub-cellular locations, one can gain spatial information within the cell by tracking organelles and proteins. The photoactivated PAmCherry can be visualized with the same filter sets used for other red fluorescent proteins. | PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350–400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag; by expressing in cells and localizing it to sub-cellular locations, one can gain spatial information within the cell by tracking organelles and proteins. The photoactivated PAmCherry can be visualized with the same filter sets used for other red fluorescent proteins. | ||
| − | [[Image: | + | [[Image:pWCD505apre2.png|400px]] [[Image:pWCD505apost2.png|400px]]<br> |
Both images are of PAmCherry expressed in the yeast cell targeted to the cytosol. The left image shows PAmCherry prior to being photoactivated. There is a diffuse low level of fluorescence before activation. The right image was taken post-activation of PAmCherry showing fluorescence magnitudes brighter. | Both images are of PAmCherry expressed in the yeast cell targeted to the cytosol. The left image shows PAmCherry prior to being photoactivated. There is a diffuse low level of fluorescence before activation. The right image was taken post-activation of PAmCherry showing fluorescence magnitudes brighter. | ||
<partinfo>BBa_K900000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K900000 SequenceAndFeatures</partinfo> | ||
Revision as of 02:55, 4 October 2012
PAmCherry (photoactivatable red fluorescent protein)
PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350–400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag; by expressing in cells and localizing it to sub-cellular locations, one can gain spatial information within the cell by tracking organelles and proteins. The photoactivated PAmCherry can be visualized with the same filter sets used for other red fluorescent proteins.
Both images are of PAmCherry expressed in the yeast cell targeted to the cytosol. The left image shows PAmCherry prior to being photoactivated. There is a diffuse low level of fluorescence before activation. The right image was taken post-activation of PAmCherry showing fluorescence magnitudes brighter.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
- 1000COMPATIBLE WITH RFC[1000]