Difference between revisions of "Part:BBa K896900:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | PCR primers (TM:55C): | ||
X174_pD_FP: gcttctag cattttgttcatggtagagattctct | X174_pD_FP: gcttctag cattttgttcatggtagagattctct | ||
X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga | X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Revision as of 03:41, 27 April 2012
pD and rbsD from ΦX174
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR primers (TM:55C):
X174_pD_FP: gcttctag cattttgttcatggtagagattctct X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga
Source
Bacteriophage ΦX174 RF DNA
References
Sorensen S. E. et al (1998) Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities. Biochem Cell Biol. 76(4):625-36.