Difference between revisions of "Part:BBa K863204:Design"

 
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<partinfo>BBa_K863204 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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the design considerations are coming soon
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This part was build by a Gibson assembly. 5' overlap parts were created by PCR with the following primers.
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The 5' UTR of alcohol oxidase 1 gene (AOX1) of Pichia pastoris [https://parts.igem.org/Part:BBa_K863200 K863200] was created with the primers:
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FW: 5'-CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAGATCTAACATCCAAAGACG-3'
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RV: 5'-GGTGGCGGCGGGCGTTTCGAATAATTAGTT-3'
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The 3' UTR of alcohol oxidase 1 gene (AOX1) of Pichia pastoris [https://parts.igem.org/Part:BBa_K863201 K863201] was created with the primers:
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FW: 5'-TCACCGCAATGCTGTTAAGGTTCGTATGGAGAAATAATAACGAGTATCTATGATTGGAAG-3'
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RV: 5'-AAAACAAGATAGTGCCCCTC-3'
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All components were created as Parts for the iGEM community:
  
 
===Source===
 
===Source===

Revision as of 03:14, 27 September 2012

shuttle vector pECPP11JS for recombination of genes of interest in yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4086
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3381
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 1532
    Illegal BamHI site found at 1889
    Illegal XhoI site found at 1196
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4086
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4242
    Illegal BsaI.rc site found at 2041


Design Notes

This part was build by a Gibson assembly. 5' overlap parts were created by PCR with the following primers.

The 5' UTR of alcohol oxidase 1 gene (AOX1) of Pichia pastoris K863200 was created with the primers:

FW: 5'-CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAGATCTAACATCCAAAGACG-3'

RV: 5'-GGTGGCGGCGGGCGTTTCGAATAATTAGTT-3'

The 3' UTR of alcohol oxidase 1 gene (AOX1) of Pichia pastoris K863201 was created with the primers:

FW: 5'-TCACCGCAATGCTGTTAAGGTTCGTATGGAGAAATAATAACGAGTATCTATGATTGGAAG-3'

RV: 5'-AAAACAAGATAGTGCCCCTC-3'






All components were created as Parts for the iGEM community:

Source

This part is made of the BioBricks XYZ by gibson assembly.

References