Difference between revisions of "Part:BBa K863203:Design"
JSchirmacher (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR. | The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR. | ||
| + | |||
| + | Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer. | ||
| + | fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGAGCTTGCTAGATTCTAATCAA-3' | ||
| + | rev: 5'-CTGCAGCGGCCGCTACTAGTATAAGCTTGCACAAACGAACT-3' | ||
===Source=== | ===Source=== | ||
Revision as of 02:49, 27 September 2012
AOX1 Terminator from Pichia pastoris
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer. fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGAGCTTGCTAGATTCTAATCAA-3' rev: 5'-CTGCAGCGGCCGCTACTAGTATAAGCTTGCACAAACGAACT-3'
Source
Pichia pastoris wild type X-33