Difference between revisions of "Part:BBa K863202:Design"

 
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<partinfo>BBa_K863202 short</partinfo>
 
<partinfo>BBa_K863202 short</partinfo>
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===Source===
 
===Source===
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Pichia pastoris wild type X-33
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The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
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===References===
 
===References===

Revision as of 02:25, 27 September 2012

his4 gene from Pichia pastoris


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 510


Design Notes

design considerations are coming soon


Source

Pichia pastoris wild type X-33

References