Difference between revisions of "Part:BBa K863201:Design"

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===Source===
 
===Source===
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The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
 
  
 
===References===
 
===References===

Revision as of 02:22, 27 September 2012

3' UTR site of alcohol oxidase 1 gene (aox1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 59


Design Notes

Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagCGAGTATCTATGATTGGAA-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTAAAAACAAGATAGTGCCCCTC-3'

Source

References