Difference between revisions of "Part:BBa K863200:Design"
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===Source=== | ===Source=== | ||
− | Pichia pastoris | + | Pichia pastoris wild type X-33 |
===References=== | ===References=== |
Revision as of 02:17, 27 September 2012
5' UTR site of alcohol oxidase 1 gene (aox1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagAGATCTAACATCCAAAGACG-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTACGTTTCGAATAATTAGTTGT-3'
Source
Pichia pastoris wild type X-33