Difference between revisions of "Part:BBa K863122"
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Cormack ''et al''.<cite>Cormack</cite> report detectable fluorescence within 8 mins. | Cormack ''et al''.<cite>Cormack</cite> report detectable fluorescence within 8 mins. | ||
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==Pictures== | ==Pictures== | ||
<gallery> | <gallery> | ||
Revision as of 22:01, 26 September 2012
BBa_I13522 with His-tag
GFP with constant promoter and His-tag made from BBa_I13522 (without the terminators).
Fluorescence wavelengths
Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -
- Excitation max - 501nm
- Emission max - 511nm
Latency
Cormack et al.Cormack report detectable fluorescence within 8 mins.
Pictures
BBa_I13522 visualized under non-UV lightbox
BBa_I13522 visualized under 254nm wavelength UV lightbox
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 813
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724