Difference between revisions of "Part:BBa K863122"
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===Usage and Biology===
 
===Usage and Biology===
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GFP (mut3b) [note that this part does not have a barcode]
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[[Help:Tag|Tag]]ged version of gfp from Repressilator reporter.  See the [[Part:BBa_E0040:Design | design page]] for more source information.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_E0040 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_E0040 parameters</partinfo>
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====Fluorescence wavelengths====
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Cormack ''et al''.<cite>Cormack</cite> report the following excitation and emission data for GFPmut3 - <br>
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*'''Excitation max''' - 501nm
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*'''Emission max''' - 511nm
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====Latency====
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Cormack ''et al''.<cite>Cormack</cite> report detectable fluorescence within 8 mins.
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''Please add maturation time data for E0040 here.''
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==Pictures==
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<gallery>
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Image:I13522 lightbox.jpg|BBa_I13522 visualized under non-UV lightbox
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Image:I13522 - UV 254nm.JPG|BBa_I13522 visualized under 254nm wavelength UV lightbox
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</gallery>
  
 
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Revision as of 22:00, 26 September 2012

BBa_I13522 with His-tag

GFP with constant promoter and His-tag made from BBa_I13522 (without the terminators).

Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

  • Excitation max - 501nm
  • Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins. Please add maturation time data for E0040 here.

Pictures

  • BBa_I13522 visualized under non-UV lightbox

  • BBa_I13522 visualized under 254nm wavelength UV lightbox

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724