Difference between revisions of "Part:BBa K863120:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg). | |
Primer that were used to made this BioBrick: | Primer that were used to made this BioBrick: | ||
Latest revision as of 22:43, 26 September 2012
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 721 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 650
Design Notes
This BioBrick is meant to be a reporter-domain. It can be fused with the Standard 25 assembly (Freiburg).
Primer that were used to made this BioBrick:
| GFP_Freiburg | ACGTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC |
| GFP_Freiburg_compl | ACGTCTGCAGCGGCCGCTACTAGTATTAACCGGTTTTGTATAGTTCATCCATGCCATGTGT |