Difference between revisions of "Part:BBa K863114:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part is was made by a mixture of Freiburg-assembly of <partinfo>BBa_K863111</partinfo> and <partinfo>BBa_K863121</partinfo> and suffix insertion into < | + | This part is was made by a mixture of Freiburg-assembly of <partinfo>BBa_K863111</partinfo> and <partinfo>BBa_K863121</partinfo> and suffix insertion into <partinfo>BBa_J61101</partinfo>. |
Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter. | Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter. | ||
Latest revision as of 21:28, 26 September 2012
CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal NgoMIV site found at 65
Illegal AgeI site found at 1109 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1020
Design Notes
This part is was made by a mixture of Freiburg-assembly of BBa_K863111 and BBa_K863121 and suffix insertion into BBa_J61101. Its purpose was to analyze of the expression problem of a similar construct with a T7-promoter.
Source
The source of the cellulose binding domain is a cloning-vector from the fermentations group of Bielefeld University. BBa_J61101 and GFP are from the parts.igem.