Difference between revisions of "Part:BBa K863113:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | CBDclos fused | + | This Biobrick is the result of a Standard 25 assembly of the BioBricks <partinfo>BBa_K863112</partinfo><partinfo>BBa_K863121</partinfo> and was designed to express the cellulose binding domain of ''Clostridium Cellulovorans'' binding protein (CBDclos) with a fused GFP as a reporter protein under the control of a T7-promoter <partinfo>BBa_K525998</partinfo>. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP. |
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===Source=== | ===Source=== |
Revision as of 20:52, 26 September 2012
Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1067
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 978
Design Notes
This Biobrick is the result of a Standard 25 assembly of the BioBricks BBa_K863112BBa_K863121 and was designed to express the cellulose binding domain of Clostridium Cellulovorans binding protein (CBDclos) with a fused GFP as a reporter protein under the control of a T7-promoter BBa_K525998. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene. A short C-terminal (Glycine, Serine) linker and the Freiburg-scar connect the CBD to GFP. For easy capturing a His-tag was added to the C-terminal end of the GFP.
Source
The origin of this part is a cloning-vector from the fermentation group of Bielefeld University (the cellulose domain) and BBa_K863121 as template for the GFP-fusion protein.