Difference between revisions of "Part:BBa K863111:Design"

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===Design Notes===
 
===Design Notes===
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The sequence starts with the rest of the Freiburg-prefix (ATG and ''Ngo''MIV-restriction-site). The A from the startcodon ATG is the last base of the ''Xba''I restriction-site of the [https://parts.igem.org/Assembly_standard_25 Standard 25 Prefix]. I isolated 12 conserved Bases (4 AS) upstream of the cellulose binding domain and 9 bases downstream of the cellulose binding protein gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.
+
The sequence starts with the rest of the Freiburg-prefix (ATG and ''Ngo''MIV-restriction-site). The A from the startcodon ATG is the last base of the ''Xba''I restriction-site of the [https://parts.igem.org/Assembly_standard_25 Standard 25 Prefix]. I isolated 12 conserved Bases (4 AS) upstream of the cellulose binding domain and 6 bases downstream of the cellulose binding protein gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.
  
 
Primers that were used to make this BioBrick:
 
Primers that were used to make this BioBrick:

Revision as of 21:52, 26 September 2012

Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence starts with the rest of the Freiburg-prefix (ATG and NgoMIV-restriction-site). The A from the startcodon ATG is the last base of the XbaI restriction-site of the Standard 25 Prefix. I isolated 12 conserved Bases (4 AS) upstream of the cellulose binding domain and 6 bases downstream of the cellulose binding protein gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.

Primers that were used to make this BioBrick:

CBDclos_Freiburg-Prefix GCTAGAATTCGCGGCCGCTTCTAGATGGCCGGCTCATCAATGTCAGTTGAATTTTAC
CBDclos_2ASlink_compl CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCTGCAAATCCAAATTCAACATATGTATC

Source

Cloning-Vector

References