Difference between revisions of "Part:BBa K863102:Design"

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(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame [https://parts.igem.org/Assembly_standard_25 Standard 25)]assembly possible.
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The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter <partinfo>BBa_K525998</partinfo>. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in-frame ([https://parts.igem.org/Assembly_standard_25 Standard 25]) assembly possible.
  
 
Primers that were used to make this BioBrick:
 
Primers that were used to make this BioBrick:

Latest revision as of 20:44, 26 September 2012

Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) under the control of a T7-promoter BBa_K525998. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in-frame (Standard 25) assembly possible.

Primers that were used to make this BioBrick:

CBDcex_T7RBS TGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAATAATGGGTCCGGCCGGGTGCCAGGT
CBDcex_2AS-Link_Frei CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCGCCGACCGTGCAGGGCGTGC

Source

Origin of the cellulose binding domain is a cloning-vector form the fermentation group of Bielefeld University.

References