Difference between revisions of "Part:BBa K863101:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | {|width='80%' style='border:1px solid gray' | ||
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| + | <partinfo>BBa_K863101 AddReview 5</partinfo> | ||
| + | <I>Imperial College 2014</I> | ||
| + | |width='60%' valign='top'| | ||
| + | We successfully used this part: We fused it to sfGFP and expressed the fusion protein and characterised it's binding ability relative to other CBDs as described below: | ||
| + | The binding ability of this CBD to bacterial cellulose was characterised when fused to [https://parts.igem.org/Part:BBa_K1321337 sfGFP], relative to other CBDs fused to [https://parts.igem.org/Part:BBa_K1321337 sfGFP]. The binding ability was represented by the percentage fluorescence remaining from the sfGFP-CBD fusions bound to bacterial cellulose discs, when subjected to various washes (protocol [http://2014.igem.org/Team:Imperial/Protocols here]). When washed with PBS, sfGFP-CBDcex fusions performed the best out of all the CBDs tested (third graph) followed by washes with 5% BSA (fourth graph), dH20 (first graph) and 70% EtOH (second graph). | ||
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| + | [[File:IC14_-_dH2Obplot1.png|700px|left|]] | ||
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| + | [[File:IC14-EtOHbplot1.png|700px|left|]] | ||
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| + | [[File:IC14-PBSbplot1.png|700px|left|]] | ||
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| + | [[File:IC14-BSAbplot1.png|700px|left|]]. | ||
| + | |}; | ||
Revision as of 04:33, 2 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K863101
User Reviews
UNIQc7ced5348d5a1187-partinfo-00000000-QINU UNIQc7ced5348d5a1187-partinfo-00000001-QINU
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Imperial College 2014 |
We successfully used this part: We fused it to sfGFP and expressed the fusion protein and characterised it's binding ability relative to other CBDs as described below: The binding ability of this CBD to bacterial cellulose was characterised when fused to sfGFP, relative to other CBDs fused to sfGFP. The binding ability was represented by the percentage fluorescence remaining from the sfGFP-CBD fusions bound to bacterial cellulose discs, when subjected to various washes (protocol [http://2014.igem.org/Team:Imperial/Protocols here]). When washed with PBS, sfGFP-CBDcex fusions performed the best out of all the CBDs tested (third graph) followed by washes with 5% BSA (fourth graph), dH20 (first graph) and 70% EtOH (second graph).     |