Difference between revisions of "Part:BBa K823040"

Latest revision as of 13:41, 26 September 2012

Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha

This part reverses the positive Input of the P_BAD Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZα Reporter, by the translational inhibitory action of the small RNA RyhB to uof_CGU translationally fused to lacZα.

This Image depicts the β-Galactosidase assay done with this part. It converts the positive Input of the P_BAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 495
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 [[Image:Inverter_pellets.jpg|600px]]
-This part reverses the positive Input of the pBad Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha.
+This part reverses the positive Input of the P<sub>''BAD''</sub> Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the ''lacZα'' Reporter, by the translational inhibitory action of the small RNA RyhB to uof<sub>CGU</sub> translationally fused to lacZα.
 [[Image:Inverter_graph.png|300px]][[Image:Inverter Construct.png|300px]]
-This Image depicts the ONPG assay done with this part. It converts the positive Input of the pBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).
+This Image depicts the β-Galactosidase assay done with this part. It converts the positive Input of the P<sub>''BAD''</sub> promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).
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