Difference between revisions of "Part:BBa K823027:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K823027 short</partinfo>
 
<partinfo>BBa_K823027 short</partinfo>
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===Source===
 
===Source===
  
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This part is an igem compatible version of the Bacillus subtilis vector pAX01
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This part is an igem compatible version of the ''Bacillus subtilis'' vector pAX01
  
 
===References===
 
===References===
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 +
[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']

Revision as of 08:50, 26 September 2012

pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 91
    Illegal NotI site found at 1622
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 2591
    Illegal NgoMIV site found at 4919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4743
    Illegal SapI site found at 3660


Design Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.


Source

This part is an igem compatible version of the Bacillus subtilis vector pAX01

References

[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl et al.]