Difference between revisions of "Part:BBa K823026:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K823026 short</partinfo>
 
<partinfo>BBa_K823026 short</partinfo>
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===Source===
 
===Source===
  
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This part is an igem compatible version of the Bacillus expression vector pDG148
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This part is an igem compatible version of the ''Bacillus subtilis'' expression vector pDG148
  
 
===References===
 
===References===
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[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']

Latest revision as of 08:48, 26 September 2012

pSBBs0K-Pspac (replicative Bacillus subtilis expression vector; IPTG inducible


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 8268
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 8274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 8268
    Illegal BglII site found at 5862
    Illegal BamHI site found at 1378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 8268
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 8268
    Plasmid lacks a suffix.
    Illegal XbaI site found at 8283
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2925
    Illegal AgeI site found at 5473
    Illegal AgeI site found at 6435
    Illegal AgeI site found at 7110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2749
    Illegal BsaI.rc site found at 4188
    Illegal BsaI.rc site found at 6704
    Illegal SapI site found at 1666
    Illegal SapI.rc site found at 5686


Design Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.


Source

This part is an igem compatible version of the Bacillus subtilis expression vector pDG148

References

[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph et al.]