Difference between revisions of "Part:BBa K823024:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823024 short</partinfo> | <partinfo>BBa_K823024 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | This part is an igem compatible version of the Bacillus expression vector pXT | + | This part is an igem compatible version of the ''Bacillus subtilis'' expression vector pXT |
===References=== | ===References=== | ||
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| + | [http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.''] | ||
Latest revision as of 08:45, 26 September 2012
pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4773
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4779 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4773
Illegal BamHI site found at 4757
Illegal XhoI site found at 1011
Illegal XhoI site found at 1929 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4773
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4773
Plasmid lacks a suffix.
Illegal XbaI site found at 4788
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1893
Illegal NgoMIV site found at 3032 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2856
Illegal SapI.rc site found at 605
Design Notes
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
Source
This part is an igem compatible version of the Bacillus subtilis expression vector pXT
References
[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré et al.]