Difference between revisions of "Part:BBa K823021:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K823021 short</partinfo>
 
<partinfo>BBa_K823021 short</partinfo>
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===Source===
 
===Source===
  
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This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
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This vector is the ''Bacillus subtilis'' vector pAC6 brought into the iGEM BioBrick standard.
  
 
===References===
 
===References===
 +
 +
[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke]

Latest revision as of 08:42, 26 September 2012

pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 9771
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 9777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 9771
    Illegal BglII site found at 8313
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 9771
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 9771
    Plasmid lacks a suffix.
    Illegal XbaI site found at 9786
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4562
    Illegal NgoMIV site found at 6888
    Illegal NgoMIV site found at 8688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4738
    Illegal SapI.rc site found at 5820


Design Notes

This reporter vector can be used with the normal BioBrick assembly strategies.


Source

This vector is the Bacillus subtilis vector pAC6 brought into the iGEM BioBrick standard.

References

[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke]