Difference between revisions of "Part:BBa K822003"
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<partinfo>BBa_K822003 short</partinfo> | <partinfo>BBa_K822003 short</partinfo> | ||
Yellow Fluorescent Protein coding sequence expressed under a constitutive promoter for use as positive control in fluorescence measurement experiments. | Yellow Fluorescent Protein coding sequence expressed under a constitutive promoter for use as positive control in fluorescence measurement experiments. | ||
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| + | Testing: | ||
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| + | The part was tested using a Tecan Infinite 200 Pro plate reader for fluorescence measurements. A culture of E. coli strain DH5a containing the part was incubated for ~24 h at 37 C with shaking. Cells containing a construct with GFP (<partinfo>BBa_K082003</partinfo>) under the Jen1 promoter (<partinfo>BBa_K284002</partinfo>) were not expected to express GFP under the conditions and were used as a negative control. | ||
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| + | [[image:YFP_fluorescence_1.png]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 12:55, 26 September 2012
Constitutive YFP generator
Yellow Fluorescent Protein coding sequence expressed under a constitutive promoter for use as positive control in fluorescence measurement experiments.
Testing:
The part was tested using a Tecan Infinite 200 Pro plate reader for fluorescence measurements. A culture of E. coli strain DH5a containing the part was incubated for ~24 h at 37 C with shaking. Cells containing a construct with GFP (BBa_K082003) under the Jen1 promoter (BBa_K284002) were not expected to express GFP under the conditions and were used as a negative control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]