Difference between revisions of "Part:BBa K822002"
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Coding sequence for the channel-forming protein Colicin E1 of E. coli and its associated immunoprotein. | Coding sequence for the channel-forming protein Colicin E1 of E. coli and its associated immunoprotein. | ||
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| + | ==Testing:== | ||
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| + | The part was placed under a constitutive promoter and cell growth experiments performed to determine if cell growth would be inhibited in samples treated with lysate of the cells containing the part. | ||
| + | Experiments showed a delayed response with differences between those samples treated with lysate from the putative colicin-producing cells and those treated with lysate from a negative control being apparent after about 3 hours. | ||
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| + | [[image:Colicin_graph1.jpg]] | ||
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| + | [[image:Colicin_graph2.png]] | ||
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Revision as of 19:25, 25 September 2012
Colicin E1 + immunoprotein
Coding sequence for the channel-forming protein Colicin E1 of E. coli and its associated immunoprotein.
Testing:
The part was placed under a constitutive promoter and cell growth experiments performed to determine if cell growth would be inhibited in samples treated with lysate of the cells containing the part. Experiments showed a delayed response with differences between those samples treated with lysate from the putative colicin-producing cells and those treated with lysate from a negative control being apparent after about 3 hours.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 301