Difference between revisions of "Part:BBa K822000"
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The promoter is repressed by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. [1]
 
The promoter is repressed by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. [1]
  
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The activity of lldP may be repressed by chloramphenicol and nutrient-rich growth media.
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The activity of the promoter may be repressed by chloramphenicol and nutrient-rich growth environment. [2]
  
 
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References:
 
References:
  
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[1]: Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997
+
[1]: Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera  
 +
et al. J. Bacteriol. 2008, 190(8):2997
 +
 
 +
[2]: Inducible Membrane-bound L-Lactate Dehydrogenase from Escherichia coli - purification and properties. Futai, M. and Kimura, H. Journal of Biological Chemistry. 1977, 252(16):5820-5827.

Revision as of 14:44, 26 September 2012

lldPRD operon promoter + RBS from E. coli

This is the promoter region for the lldPRD operon (previously named lct) of E. coli. The lldPRD operon encodes a lactate-dehydrogenase, and the lldPRD promoter has been shown to be induced by L-lactate.

The promoter is repressed by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. [1]

The activity of the promoter may be repressed by chloramphenicol and nutrient-rich growth environment. [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 335


References:

[1]: Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997

[2]: Inducible Membrane-bound L-Lactate Dehydrogenase from Escherichia coli - purification and properties. Futai, M. and Kimura, H. Journal of Biological Chemistry. 1977, 252(16):5820-5827.