Difference between revisions of "Part:BBa K822000"

 
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__NOTOC__
 
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<partinfo>BBa_K822000 short</partinfo>
 
<partinfo>BBa_K822000 short</partinfo>
  
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lldP is a promoter associated with the lldPRD operon of E. coli. The lldPRD operon encodes a lactate-dehydrogenase, and the lldP promoter has been shown to be induced by L-lactate.  
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This is the promoter region for the lldPRD operon (previously named lct) of E. coli. The lldPRD operon encodes a lactate-dehydrogenase, and the lldPRD promoter has been shown to be induced by L-lactate.  
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The promoter is repressed by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. [1]
  
 
The activity of lldP may be repressed by chloramphenicol and nutrient-rich growth media.
 
The activity of lldP may be repressed by chloramphenicol and nutrient-rich growth media.
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===Usage and Biology===
 
===Usage and Biology===
  
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The promoter is inhbited by the ArcA/ArcB system under low-oxygen conditions.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K822000 parameters</partinfo>
 
<partinfo>BBa_K822000 parameters</partinfo>
 
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References:
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[1]: Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997

Revision as of 13:09, 23 September 2012

lldPRD operon promoter + RBS from E. coli

This is the promoter region for the lldPRD operon (previously named lct) of E. coli. The lldPRD operon encodes a lactate-dehydrogenase, and the lldPRD promoter has been shown to be induced by L-lactate.

The promoter is repressed by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. [1]

The activity of lldP may be repressed by chloramphenicol and nutrient-rich growth media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 335


References:

[1]: Dual role of lldR in regulation of the lldPRD operon, involved in L-lactate metabolism in E. coli. Aguilera et al. J. Bacteriol. 2008, 190(8):2997