Difference between revisions of "Part:BBa K814009"
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This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli. | This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli. | ||
+ | Balskus and Walsh, 2009. | ||
+ | |||
+ | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766776/] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 22:50, 13 October 2012
ScyC generator
Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyC (NpR1276) is a protein of unknown function which converts the ketoacid generated by ScyA into an indole compound which spontaneously dimerizes to form scytonemin.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyC. This part can be used to express ScyC protein in E. coli.
Balskus and Walsh, 2009.
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766776/]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2015
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 134
Illegal XhoI site found at 2023 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 794
- 1000COMPATIBLE WITH RFC[1000]