Difference between revisions of "Part:BBa K814007"
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This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyA. This part can be used to express ScyA protein in E. coli.
 
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyA. This part can be used to express ScyA protein in E. coli.
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[[Image:Abcuv.png]]
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Figure 1. The scytonemin biosynthetic pathway showing the BioBrick stacking of ScyA. 
  
  

Revision as of 22:47, 13 October 2012

ScyA (acetolactate synthase) generator

Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyA (NpR1276), an acetolactate synthetase, carries out the second step in the pathway by converting 3-indole pyruvic acid to a ketoacid intermediate.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyA. This part can be used to express ScyA protein in E. coli.


Abcuv.png

Figure 1. The scytonemin biosynthetic pathway showing the BioBrick stacking of ScyA.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 743
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 743
    Illegal NotI site found at 1109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 743
    Illegal BglII site found at 134
    Illegal XhoI site found at 1117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 743
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]