Difference between revisions of "Part:BBa K814006"

 
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<partinfo>BBa_K814006 short</partinfo>
 
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This composite part contains protein generators for DHQS and ATPG.  The part is non-functional and was used by the 2012 Minnesota iGEM team as a negative control when testing biosynthesis of mycosporine-like amino acids by the parts BBa_K814004 and BBa_K814005.
 
This composite part contains protein generators for DHQS and ATPG.  The part is non-functional and was used by the 2012 Minnesota iGEM team as a negative control when testing biosynthesis of mycosporine-like amino acids by the parts BBa_K814004 and BBa_K814005.
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[[Image:hplcuv.png]]
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Figure 1. HPLC trace showing the DA negative control lacking production of UV-protectant compounds.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:42, 13 October 2012

negative control for mycosporine-like amino acid biosynthesis

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate. Dehydroquinate-O-methyltransferase (O-MT) then generates 4-deoxygadusol, which is converted to mycosprine-glycine by ATP-grasp (ATPG). Finally, mycosporine-glycine is converted into shinorine by a non-ribosomal peptide synthase (NRPS).

This composite part contains protein generators for DHQS and ATPG. The part is non-functional and was used by the 2012 Minnesota iGEM team as a negative control when testing biosynthesis of mycosporine-like amino acids by the parts BBa_K814004 and BBa_K814005.

Hplcuv.png

Figure 1. HPLC trace showing the DA negative control lacking production of UV-protectant compounds.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal NotI site found at 1373
    Illegal NotI site found at 2970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
    Illegal BglII site found at 134
    Illegal BglII site found at 1587
    Illegal XhoI site found at 1381
    Illegal XhoI site found at 2978
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
    Illegal EcoRI site found at 2397
  • 1000
    COMPATIBLE WITH RFC[1000]