Difference between revisions of "Part:BBa K814001"
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Figure 1.  PCR screen of ATP-grasp confirming successful cloning of this ORF into the pUCBB plasmid.
 
Figure 1.  PCR screen of ATP-grasp confirming successful cloning of this ORF into the pUCBB plasmid.
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Balskus and Walsh, 2010. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116657/]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:53, 13 October 2012

ATP-grasp (ATPG) generator

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. ATP-grasp (ATPG) catalyzes the third step in this pathway, 4-deoxygadusol into mycosporine-glycine.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. Dhqs.png

Figure 1. PCR screen of ATP-grasp confirming successful cloning of this ORF into the pUCBB plasmid.

Balskus and Walsh, 2010. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116657/]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 944
    Illegal NotI site found at 1517
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 944
    Illegal BglII site found at 134
    Illegal XhoI site found at 1525
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 944
  • 1000
    COMPATIBLE WITH RFC[1000]