Difference between revisions of "Part:BBa K814001"
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This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. | This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli. | ||
+ | [[Image:Dhqs.png]] | ||
+ | Figure 1. PCR screen of ATP-grasp confirming successful cloning of this ORF into the pUCBB plasmid. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 22:28, 13 October 2012
ATP-grasp (ATPG) generator
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. ATP-grasp (ATPG) catalyzes the third step in this pathway, 4-deoxygadusol into mycosporine-glycine.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.
Figure 1. PCR screen of ATP-grasp confirming successful cloning of this ORF into the pUCBB plasmid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 944
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 944
Illegal NotI site found at 1517 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 944
Illegal BglII site found at 134
Illegal XhoI site found at 1525 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 944
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 944
- 1000COMPATIBLE WITH RFC[1000]