Difference between revisions of "Part:BBa K792004"
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Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process ''[Derossi 1998]''. They '''can be used to increase the efficiency with which a protein enters a cell'''.
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Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process. They '''can be used to increase the efficiency with which a protein enters a cell'''.
  
  

Revision as of 19:23, 25 September 2012

Import enhancer - Polyarginine (trojan peptide)


Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process. They can be used to increase the efficiency with which a protein enters a cell.


This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.


This part includes a HindIII restriction site as an extra.


(Refer to Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] for more information about polyarginine)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]