Difference between revisions of "Part:BBa K658016:Design"

(Design Notes)
Line 10: Line 10:
 
The device is constructed by combining two parts.
 
The device is constructed by combining two parts.
  
XXXXXXXXXXXXXXXXXXXXX Fig.1 XXXXXXXXXXXXXXXXXX
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[[Image:XMU_China_k658016F1.jpg]]
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg">
 +
</html>
  
 
The two parts are proven to be right.  
 
The two parts are proven to be right.  
 
+
[[Image:XMU_China_k658016F2.jpg]]
XXXXXXXXXXXXXXXXXXXXX Fig.2 XXXXXXXXXXXXXXXXXX
+
<html>
 +
<img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg">
 +
</html>
  
 
From the electrophoretogram,we can see that the part is successfully constructed.
 
From the electrophoretogram,we can see that the part is successfully constructed.

Revision as of 03:05, 6 October 2011

LuxI->LuxR->lux pR->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1874
    Illegal BsaI.rc site found at 2601


Design Notes

Similar to the definition of RBS strength, the strength of promoters lux pR and its three mutants could be tested using this device.

The device is constructed by combining two parts.

XMU China k658016F1.jpg

The two parts are proven to be right. XMU China k658016F2.jpg

From the electrophoretogram,we can see that the part is successfully constructed.

Source

K658012


References