Difference between revisions of "Part:BBa K658003:Design"
(→Design Notes) |
|||
Line 12: | Line 12: | ||
2) LuxR producer | 2) LuxR producer | ||
+ | [[Image:XMU_China_k658003f1.jpg|left]] | ||
+ | <html> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
− | |||
3)killer protein producer | 3)killer protein producer | ||
− | + | [[Image:XMU_China_k658003f2.jpg|left]] | |
+ | <html> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
+ | |||
The device is successfully constructed which can be verified by the restriction analysis shown as follows: | The device is successfully constructed which can be verified by the restriction analysis shown as follows: | ||
− | + | [[Image:XMU_China_k658003f3.jpg|left]] | |
+ | <html> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
+ | |||
===Source=== | ===Source=== | ||
===References=== | ===References=== |
Revision as of 03:07, 6 October 2011
a bacteria population-control device with RBS0.6 driven by lacl+pL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2347
Illegal BsaI.rc site found at 1874
Design Notes
The part was designed to combine three parts together to construct a population-control device.The three subparts are a LuxI producer, a LuxR producer and a killer protein producer.
1) LuxI producer
2) LuxR producer
3)killer protein producer
The device is successfully constructed which can be verified by the restriction analysis shown as follows: