Difference between revisions of "Part:BBa K575033"

Revision as of 20:46, 28 September 2011

This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI2 (C4-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI2 from the environment and the RhlR receptor (produced by this construct). As the graph shows, addition of the autoinducer produces a significant difference in fluorescence compared to the controls. Thus, our construct is successful both in production of RhlR and transcription of GFP in the presence of PAI2.

[Image:rhlp-30-gfp_cp-34-rhlr.jpg]

RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0034) + RhlR

Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 817
Illegal NheI site found at 840
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1111
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1586
Illegal BsaI.rc site found at 726

@@ Line 1: / Line 1: @@
-This construct is designed to report the presence of PAI2, part of the ''Pseudomonas'' quorum sensing hierarchy. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.
+This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI2 (C4-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI2 from the environment and the RhlR receptor (produced by this construct). As the graph shows, addition of the autoinducer produces a significant difference in fluorescence compared to the controls. Thus, our construct is successful both in production of RhlR and transcription of GFP in the presence of PAI2.
-[[Image:rhlp-30-gfp_cp-34-rhlr.jpg]]
+[Image:rhlp-30-gfp_cp-34-rhlr.jpg]
 __NOTOC__