Difference between revisions of "Part:BBa K575028"
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Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter. | Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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[[Image:Standardized Fluorescence for S2.jpg]]__NOTOC__ | [[Image:Standardized Fluorescence for S2.jpg]]__NOTOC__ | ||
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| + | When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 20:24, 24 September 2011
LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0030) + LasR
Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.
When induced with PAI-1 (3-oxo-C12-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
Illegal NheI site found at 918
Illegal NheI site found at 941 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1297
Illegal AgeI site found at 1494 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 827