Difference between revisions of "Part:BBa K575008:Design"

 
(Design Notes)
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<partinfo>BBa_K575008 short</partinfo>
 
<partinfo>BBa_K575008 short</partinfo>
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===Design Notes===
 
===Design Notes===
Need to ensure no leaking transcription of GFP.
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Some leaking transcription of GFP, even when the promoter is not induced. However, it is significantly less than when the promoter is induced.
 
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===Source===
 
===Source===

Revision as of 21:10, 24 September 2011

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830


Design Notes

Some leaking transcription of GFP, even when the promoter is not induced. However, it is significantly less than when the promoter is induced.

Source

From Biobrick parts registry

References