Difference between revisions of "Part:BBa K546546:Design"

 
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===Design Notes===
 
===Design Notes===
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Some parameters of the signalling molecule formation and degradation were unknown. Modeling of the BioBrick device was therefore hard. The addtion of extra promoters should enable stablization of the oscillation.
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Some parameters of the signalling molecule formation and degradation were unknown. Modeling of the BioBrick device was therefore hard. The hybrid promoters were installed to stablize the oscillation in case it would be necessary.
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===Source===
 
===Source===

Revision as of 08:25, 18 September 2011

The Synchroscillator: A Tunable Synchronized Oscillatory System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2781
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 2042
    Illegal BsaI.rc site found at 2930
    Illegal BsaI.rc site found at 3657


Design Notes

Some parameters of the signalling molecule formation and degradation were unknown. Modeling of the BioBrick device was therefore hard. The hybrid promoters were installed to stablize the oscillation in case it would be necessary.

Source

The lux pR promoter originates from the quorum sensing ability of Vibrio fischeri.

References